Engineered Fluorescent Carbon Dots and G4-G6 PAMAM Dendrimer Nanohybrids for Bioimaging and Gene Delivery

Carbon dots (CDs) and G4-G6 (polyamidoamine)PAMAM-NH2 dendrimers were self-assembled to produce CDs@PAMAM nanohybrids for transfection bioimaging purposes. CDs synthesized by the hydrothermal method, using ascorbic acid as a starting precursor characterized transmission electron microscopy, UV–Vis, fluorescence (in solution solid-state) techniques. electrostatically combined with PAMAM at room temperature, fluorescence, NMR spectroscopies used confirm self-assembly. When compared pristine CDs, more photostable, resisting high acidic basic pH. Moreover, they considerably internalized cells, assessed flow cytometry and, when excited, displayed multi-color emission easily quantified visualized. These nanoscale hybrids, coined hybridplexes, can condense pDNA transfecting cells successfully, particularly G5 nanohybrids. In summary, prepared in mild smooth lab conditions, showing good optical properties, prepare elegantly promising biomedical applications.